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Goat anti-Human lambda Light Chain (Lambda-IgLC) Antibody (HRP)

Rockland 609-1311 Lambda-IgLC WB, IHC, ELISA Polyclonal IgG HRP
Rockland
Catalog No. ABIN5608136
Supplier Product No.: 609-1311
  • Target See all Lambda-IgLC products
    Lambda-IgLC (lambda Light Chain (Lambda-IgLC))
    Reactivity
    • 31
    • 11
    Human
    Host
    • 20
    • 12
    • 9
    • 1
    Goat
    Clonality
    • 29
    • 13
    Polyclonal
    Conjugate
    • 10
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    HRP
    Application
    • 27
    • 22
    • 21
    • 20
    • 10
    • 10
    • 9
    • 9
    • 7
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA
    Supplier Product No.
    609-1311
    Supplier
    Rockland
    Purpose
    Human (lambda chain) Antibody Peroxidase Conjugated
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. Specificity was confirmed by ELISA minimal cross reactivity against other human heavy or light chain isotypes.
    Characteristics
    The anti-Human κ (kappa chain) Antibody detects the kappa chain subunit. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). In humans the light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Human IgG kappa is conjugated to HRP.
    Purification
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    Immunogen

    Immunogen: Anti-Human lambda chain was produced by repeated immunization with human lambda light chain in goat.

    Immunogen Type: Native Protein

    Isotype
    IgG
  • Application Notes
    Application Note: Anti-Human λ (lambda chain) Antibody peroxidase conjugated has been tested by ELISA and western blot and is assayed against 1.0 μg of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:16,000 to 1:64,000 is suggested for this product. This secondary peroxidase conjugated antibody anti-Human lambda chain is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:10,000 - 1:50,000 Other: User Optimized
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    , Preservative:0.01 % (w/v) Thimerosal
    Preservative
    Thimerosal (Merthiolate)
    Precaution of Use
    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    Lambda-IgLC (lambda Light Chain (Lambda-IgLC))
    Alternative Name
    lambda light chain (Lambda-IgLC Products)
    Background
    The anti-Human λ (lambda chain) Antibody detects the lambda chain subunit. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). In humans the light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Human IgG lambda is conjugated to peroxidase.
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