Goat anti-Human lambda Light Chain (Lambda-IgLC) Antibody

Catalog No. ABIN5608137

Supplier Product No.: 609-1111

Quick Overview for Goat anti-Human lambda Light Chain (Lambda-IgLC) Antibody (ABIN5608137)

Target: Lambda-IgLC (lambda Light Chain (Lambda-IgLC))

Clonality: Polyclonal

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Isotype: IgG

Product Details

Reactivity: Human

Host: Goat

Supplier Product No.: 609-1111

Supplier: Rockland

Purpose: Human (lambda chain) Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum.  Solid phase adsorption(s) against a panel of Human IgG Kappa, IgM Kappa and IgA Kappa proteins was performed.  Specificity was confirmed by ELISA.

Characteristics: The anti-Human κ (kappa chain) Antibody detects the kappa chain subunit. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). In humans the light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-Human lambda light chain antibody was produced by repeated immunization with Human lambda light chain molecule in goat.

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Human λ has been tested by ELISA and western blot and is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:4,000 - 1:20,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Stabilizer: None

, Preservative:0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Expiry Date: 12 months

Target Details

Target: Lambda-IgLC (lambda Light Chain (Lambda-IgLC))

Alternative Name: lambda light chain

Background: The anti-Human λ (lambda chain) Antibody detects the lambda chain subunit. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). In humans the light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain.