Goat anti-Rat IgA Antibody

Catalog No. ABIN5608139

Supplier Product No.: 612-1106

Quick Overview for Goat anti-Rat IgA Antibody (ABIN5608139)

Target: IgA

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Isotype: IgG

Product Details

Reactivity: Rat

Host: Goat

Supplier Product No.: 612-1106

Supplier: Rockland

Purpose: Rat IgA (alpha chain) Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgA and Rat Serum.  No reaction was observed against Rat IgM or Rat IgG.   Specificity was confirmed by ELISA at less than 1 % cross reactivity against other Rat heavy or light chain isotypes.

Characteristics: Anti-Rat IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH .

Purification: Anti-Rat IgA was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Optional[Immunogen]: Rat IgA alpha heavy chain

Application Details

Application Notes: Application Note: Anti-Rat IgA antibody is suitable for Elisa, Western Blots and Immunohistochemistry. Antibody should be optimized by the end user for specific conditions of reactivity. Immunohistochemistry Dilution: 1:500 - 1:3,000 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:10,000 - 1:50,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Expiry Date: 12 months

Target Details

Target: IgA

Target Type: Antibody

Background: Rat IgA antibodies detects the alpha chain of the rat IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgA and Rat Serum. No reaction was observed against Rat IgM or Rat IgG. Specificity was confirmed by ELISA at less than 1 % cross reactivity against other Rat heavy or light chain isotypes. Anti-Rat IgA antibody is ideal for investigators involved in serum component research.