Goat anti-Rat IgA, IgG, IgM Antibody (TRITC)

Catalog No. ABIN5608162

Supplier Product No.: 612-100-130

Quick Overview for Goat anti-Rat IgA, IgG, IgM Antibody (TRITC) (ABIN5608162)

Target: IgA, IgG, IgM

Clonality: Polyclonal

Conjugate: TRITC

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM), FLISA

Isotype: IgG

Product Details

Reactivity: Rat

Host: Goat

Supplier Product No.: 612-100-130

Supplier: Rockland

Purpose: Rat IgG IgA IgM (H&L) Antibody Rhodamine Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum.  This reagent is suitable for the detection of all Rat immunoglobulin isotypes and chain combinations.

Characteristics: Anti-Rat IgG (H&L) rhodamine Antibody generated in goat detects reactivity to Rat IgG.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: Optional[Immunogen]: Rat IgG, IgA and IgM whole molecules

Application Details

Application Notes: Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: IgA, IgG, IgM

Alternative Name: IgG,IgA,IgM

Target Type: Antibody

Background: Anti-Rat IgG IgA IgM (H&L) Antibody generated in goat detects rat (heavy and light chain) immunoglobulin G, A, and M. Immunoglobulin G binds to antigens and can neutralize or opsonize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rat IgG IgA IgM Antibody is conjugated to rhodamine.