Rabbit anti-Mouse lambda Light Chain (Lambda-IgLC) Antibody

Catalog No. ABIN5608186

Supplier Product No.: 610-4111

Quick Overview for Rabbit anti-Mouse lambda Light Chain (Lambda-IgLC) Antibody (ABIN5608186)

Target: Lambda-IgLC (lambda Light Chain (Lambda-IgLC))

Clonality: Polyclonal

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Rabbit

Supplier Product No.: 610-4111

Supplier: Rockland

Purpose: Mouse (lambda chain) Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.  Solid phase adsorption(s) against a panel of Mouse IgG kappa, IgM kappa and IgA kappa proteins was performed.  Specificity was confirmed by ELISA.

Characteristics: Anti-Mouse κ (kappa chain) (RABBIT) Antibody generated in rabbit detects specifically Mouse light chain. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). The light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Optional[Immunogen]: Mouse lambda light chain

Application Details

Application Notes: Application Note: Anti-Mouse lambda chain antibody has been tested by ELISA and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:500 - 1:3,000 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:40,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Expiry Date: 12 months

Target Details

Target: Lambda-IgLC (lambda Light Chain (Lambda-IgLC))

Alternative Name: lambda light chain

Background: Anti-Mouse λ (lambda chain) (RABBIT) Antibody generated in rabbit detects specifically Mouse lambda light chain. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). The light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain.