Mouse anti-Human IgA (Free & Bound), (Secretory Component) Antibody (HRP)

Catalog No. ABIN567758

Quick Overview for Mouse anti-Human IgA (Free & Bound), (Secretory Component) Antibody (HRP) (ABIN567758)

Target: IgA

Clonality: Monoclonal

Conjugate: HRP

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Haemagglutination (H), Aggregation (AGG)

Clone: NI 194-4 (A89-039)

Isotype: IgG1

Product Details

Binding Specificity: Free & Bound, Secretory Component

Reactivity: Human

Host: Mouse

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Molar Ratio: Peroxidase/IgG: aprox. 1.7

Immunogen: Highly purified Secretory component isolated from Human milk.

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 0.5 mL sterile distilled water. Dilutions may be prepared by adding PBS, pH 7.2

Concentration: 0.4 mg/mL

Buffer: PBS, pH 7.2 without preservatives. No foreign protein added

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for up to one week or (in aliquots) at -28 °C for longer.

Target Details

Target: IgA

Target Type: Antibody

Background: Secretory Component Glycoprotein is an epithelial transport receptor for polymeric IgA and IgM. Antibody to secretory component has been shown to be useful for identifying glandular carcinomas. Defects in Secretory Component Glycoprotein are the cause of lipoid proteinosis (LiP), also known as lipoid proteinosis of Urbach and Wiethe or hyalinosis cutis et mucosae. LiP is a rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane. Examples of positive human tissues: secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland.Synonyms: Human Immunoglobulin A