Mouse anti-Human IgA (Free & Bound), (Secretory Component) Antibody

Catalog No. ABIN567760

Quick Overview for Mouse anti-Human IgA (Free & Bound), (Secretory Component) Antibody (ABIN567760)

Target: IgA

Clonality: Monoclonal

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: NI 194-4 (A89-039)

Isotype: IgG1

Product Details

Binding Specificity: Free & Bound, Secretory Component

Reactivity: Human

Host: Mouse

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ascites

Immunogen: Highly purified Secretory component isolated from Human milk.

Application Details

Application Notes: General Recommended Dilutions:
Histochemical Use: from 1/50-1/200.
ELISA: form 1/500 upwards.
Western blot: from 1/1000 upwards.


Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore by adding 0.5 mL sterile distilled water. Dilutions may be prepared by adding PBS, pH 7.2

Concentration: 1.0 mg/mL

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.

Target Details

Target: IgA

Target Type: Antibody

Background: Secretory Component Glycoprotein is an epithelial transport receptor for polymeric IgA and IgM. Antibody to secretory component has been shown to be useful for identifying glandular carcinomas. Defects in Secretory Component Glycoprotein are the cause of lipoid proteinosis (LiP), also known as lipoid proteinosis of Urbach and Wiethe or hyalinosis cutis et mucosae. LiP is a rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane. Examples of positive human tissues: secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland.Synonyms: Human Immunoglobulin A