Donkey anti-Sheep IgG (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN611874

Quick Overview for Donkey anti-Sheep IgG (Heavy & Light Chain) Antibody (HRP) (ABIN611874)

Target: IgG

Fragment: F(ab')2 fragment

Conjugate: HRP

Application: Immunoassay (IA)

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Sheep

Host: Donkey

Characteristics: Donkey anti-Sheep IgG (H&L) - F(ab) '2 fragment, HRP conjugate.

Purification: Affinity purification

Purity: > 95 % based on SDS-PAGE

Immunogen: Purified Sheep IgG, whole molecule

Application Details

Application Notes: This conjugate is suitable for all all immunoassays applications.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:500-1:5,000 for Immunohistochemistry, 1:200-1:5000 for ELISA/Western blot

Comment:

Country of Origin: Donkey serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 1.5 mg/mL

Buffer: Lyophilized. 0.1 % (v/v) Kathon CG.

Preservative: Kathon CG

Handling Advice: Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C

Storage Comment: Store freeze dried powder at 2-8 °C.

References

Yamane, Katayama, Tsuruo: "The BRCT regions of tumor suppressor BRCA1 and of XRCC1 show DNA end binding activity with a multimerizing feature." in: Biochemical and biophysical research communications, Vol. 279, Issue 2, pp. 678-84, (2001) (PubMed).

Yamane, Tsuruo: "Conserved BRCT regions of TopBP1 and of the tumor suppressor BRCA1 bind strand breaks and termini of DNA." in: Oncogene, Vol. 18, Issue 37, pp. 5194-203, (1999) (PubMed).

Yamane, Kawabata, Tsuruo: "A DNA-topoisomerase-II-binding protein with eight repeating regions similar to DNA-repair enzymes and to a cell-cycle regulator." in: European journal of biochemistry / FEBS, Vol. 250, Issue 3, pp. 794-9, (1998) (PubMed).

Target Details

Target: IgG

Target Type: Antibody