Goat anti-Human IgG (Heavy & Light Chain) Antibody (DyLight 650)

Catalog No. ABIN612018

Quick Overview for Goat anti-Human IgG (Heavy & Light Chain) Antibody (DyLight 650) (ABIN612018)

Target: IgG

Clonality: Polyclonal

Conjugate: DyLight 650

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Human

Host: Goat

Purpose: Goat anti-Human IgG (H&L)- Affinity Pure, DyLight ®650 Conjugate

Specificity: Based on IEP, this antibody reacts with: • heavy (i³) chains on human IgG • light chains on all human immunoglobulins

Cross-Reactivity (Details): Based on IEP, no reactivity is observed to: • non-immunoglobulin human serum immunoglobulins

Purification: Affinity purified using solid phase Human IgG

Purity: Affinity purified antibody is > 95 % based on SDS-PAGE. E 1 % at 280 nm = 13.0

Immunogen: Purified Human IgG, whole molecule

Application Details

Comment:

Country of Origin: Goat serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Concentration: 1.0 mg/mL

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free, contains 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Product is stable for up to 4 weeks at 2-8 °C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20 °C.

Storage: 4 °C

Storage Comment: Store freeze-dried powder at 2-8 °C.

Expiry Date: 4 weeks

References

Aguilar, Boehm, Gorshkova, Crouch, Tomita, Saito, Ohno, Bonifacino: "Signal-binding specificity of the mu4 subunit of the adaptor protein complex AP-4." in: The Journal of biological chemistry, Vol. 276, Issue 16, pp. 13145-52, (2001) (PubMed).

DellAngelica, Mullins, Bonifacino: "AP-4, a novel protein complex related to clathrin adaptors." in: The Journal of biological chemistry, Vol. 274, Issue 11, pp. 7278-85, (1999) (PubMed).

Hirst, Bright, Rous, Robinson: "Characterization of a fourth adaptor-related protein complex." in: Molecular biology of the cell, Vol. 10, Issue 8, pp. 2787-802, (1999) (PubMed).

Target Details

Target: IgG

Target Type: Antibody