Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (DyLight 550)

Catalog No. ABIN612393

Quick Overview for Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (DyLight 550) (ABIN612393)

Target: IgG

Clonality: Polyclonal

Conjugate: DyLight 550

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Rabbit

Host: Goat

Purpose: Goat anti-Rabbit IgG (H&L) - Affinity Pure, DyLight ®550 Conjugate, min x w/human serum proteins

Specificity: Based on IEP, this antibody reacts with: • heavy (i³) chains on rabbit IgG • light chains on all rabbit immunoglobulins

Cross-Reactivity (Details): Based on IEP, no reactivity is observed to: • non-immunoglobulin rabbit serum proteins • human serum proteins

Purification: Affinity purified using solid phase Rabbit IgG

Purity: Affinity purified antibody is > 95 % based on SDS-PAGE. E 1 % at 280 nm = 13.0

Immunogen: Purified Rabbit IgG, whole molecule

Application Details

Comment:

Country of Origin: Goat serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Concentration: 1.0 mg/mL

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free, contains 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Product is stable for up to 4 weeks at 2-8 °C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20 °C.

Storage: 4 °C

Storage Comment: Store freeze-dried powder at 2-8 °C.

Expiry Date: 4 weeks

References

Michell, Chen Zp, Tiganis, Stapleton, Katsis, Power, Sim, Kemp: "Coordinated control of endothelial nitric-oxide synthase phosphorylation by protein kinase C and the cAMP-dependent protein kinase." in: The Journal of biological chemistry, Vol. 276, Issue 21, pp. 17625-8, (2001) (PubMed).

Dimmeler, Fleming, Fisslthaler, Hermann, Busse, Zeiher: "Activation of nitric oxide synthase in endothelial cells by Akt-dependent phosphorylation." in: Nature, Vol. 399, Issue 6736, pp. 601-5, (1999) (PubMed).

Gallis, Corthals, Goodlett, Ueba, Kim, Presnell, Figeys, Harrison, Berk, Aebersold, Corson: "Identification of flow-dependent endothelial nitric-oxide synthase phosphorylation sites by mass spectrometry and regulation of phosphorylation and nitric oxide production by the phosphatidylinositol 3-kinase inhibitor LY294002." in: The Journal of biological chemistry, Vol. 274, Issue 42, pp. 30101-8, (1999) (PubMed).

Target Details

Target: IgG

Target Type: Antibody