Mouse anti-Human IgG Antibody (CF®405S)

Catalog No. ABIN6154783

Quick Overview for Mouse anti-Human IgG Antibody (CF®405S) (ABIN6154783)

Target: IgG

Clonality: Monoclonal

Conjugate: CF®405S

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: B33-20

Isotype: IgG1 kappa

Product Details

Reactivity: Human

Host: Mouse

Purpose: Mouse Monoclonal anti-Human IgG Immunoglobulin (B33/20), CF405S Conjugate

Characteristics: This antibody recognizes a protein of 75 kDa, identified as γ heavy chain of human immunoglobulins. Its epitope maps in CH2 domain of Fc region of IgG. It reacts with all sub-classes of γ chain of human immunoglobulins. It does not cross-react with α (IgA), μ (IgM), ε (IgE), or δ (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin?s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified polyclonal human Ig Gamma Chain

Application Details

Application Notes: Immunohistology formalin/paraffin 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

293T, Raji or hPBL cells. Tonsil or Spleen

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details

Target: IgG

Alternative Name: IgG Immunoglobulin

Target Type: Antibody

Molecular Weight: 75 kDa

Gene ID: 3500, 3501, 3502

UniProt: P01857, P01859, P01860, P01861