Mouse anti-Human IgM Antibody (CF®488A)

Catalog No. ABIN6154957

Quick Overview for Mouse anti-Human IgM Antibody (CF®488A) (ABIN6154957)

Target: IgM

Clonality: Monoclonal

Conjugate: CF®488A

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: DA4-4

Isotype: IgG1 kappa

Product Details

Reactivity: Human

Host: Mouse

Purpose: Mouse Monoclonal anti-Human IgM Immunoglobulin (DA4-4), CF488A Conjugate

Characteristics: This antibody recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Heavy chain of human IgM

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

293T, Raji or hPBL cells. Tonsil or Spleen

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details

Target: IgM

Alternative Name: IgM Immunoglobulin

Target Type: Antibody

Background: AGM1, IGHM, Constant Region of Heavy Chain of IgM, Ig Mu Chain C Region

Molecular Weight: 50-75 kDa

Gene ID: 3507, 510635