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Mouse anti-Human IgA Secretory Component Antibody (CF®594)

FACS, IF, IHC (f) Monoclonal ECM1-792 IgG1 kappa CF®594
Catalog No. ABIN6155149
  • Target
    IgA Secretory Component
    Reactivity
    • 11
    • 7
    • 1
    Human, Rat
    Host
    • 8
    • 4
    Mouse
    Clonality
    • 8
    • 4
    Monoclonal
    Conjugate
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    CF®594
    Application
    • 7
    • 7
    • 7
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Purpose
    Mouse Monoclonal anti-IgA Secretory Component (ECM1/792), CF594 Conjugate
    Characteristics
    This MAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Recombinant human ECM1 protein
    Clone
    ECM1-792
    Isotype
    IgG1 kappa
  • Application Notes
    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Optimal dilution for a specific application should be determined by user
    Comment

    Stomach, Lung, or breast tumor

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Protect from light
  • Target
    IgA Secretory Component
    Background
    ECM1, Extracellular Matrix Protein 1, Secretory Component p85, URBWD
    Molecular Weight
    ~80 kDa
    Gene ID
    1893, 81071
    UniProt
    Q16610
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