Mouse anti-Human IgA Secretory Component Antibody

Catalog No. ABIN6155162

Quick Overview for Mouse anti-Human IgA Secretory Component Antibody (ABIN6155162)

Target: IgA Secretory Component

Clonality: Monoclonal

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: ECM1-792

Isotype: IgG1 kappa

Product Details

Reactivity: Human, Rat

Host: Mouse

Purpose: Mouse Monoclonal anti-IgA Secretory Component (ECM1/792), Purified, BSA-free

Characteristics: This MAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Purification: Purified

Immunogen: Recombinant human ECM1 protein

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Comment:

Stomach, Lung, or breast tumor

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Preservative: Azide free

Target Details

Target: IgA Secretory Component

Background: ECM1, Extracellular Matrix Protein 1, Secretory Component p85, URBWD

Molecular Weight: ~80 kDa

Gene ID: 1893, 81071

UniProt: Q16610