Recombinant Rabbit anti-Human IgG Antibody (CF®647)

Catalog No. ABIN6170848

Quick Overview for Recombinant Rabbit anti-Human IgG Antibody (CF®647) (ABIN6170848)

Target: IgG

Antibody Type: Recombinant Antibody

Clonality: Monoclonal

Conjugate: CF®647

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: IG1707R

Isotype: IgG kappa

Product Details

Reactivity: Human

Host: Rabbit

Purpose: Recombinant Rabbit Monoclonal anti-Human IgG (IG1707R), CF647 Conjugate

Characteristics: This antibody recognizes a protein of 75 kDa, identified as gamma heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified human Ig Gamma Chain

Application Details

Application Notes: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

293T, Raji or hPBL cells. Tonsil or Spleen

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details

Target: IgG

Target Type: Antibody

Molecular Weight: 75 kDa

Gene ID: 510635