Mouse anti-Human kappa Light Chain Antibody (CF®405S)

Catalog No. ABIN6173956

Quick Overview for Mouse anti-Human kappa Light Chain Antibody (CF®405S) (ABIN6173956)

Target: kappa Light Chain

Clonality: Monoclonal

Conjugate: CF®405S

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: HP6053

Isotype: IgG1 kappa

Product Details

Reactivity: Human

Host: Mouse

Purpose: Mouse Monoclonal anti-Human Kappa Light Chain (HP6053), CF405S Conjugate

Characteristics: This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified human Ig kappa chain

Application Details

Application Notes: Immunohistology formalin-fixed 0.5-1.0 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Western blotting 0.5-1.0 μg/mL
• Flow Cytometry 0.5-2.0 μg/million cells in 0.1 mL
• Optimal dilution for a specific application should be determined by user

Comment:

293T, Raji or hPBL cells. Tonsil or Spleen

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Target Details

Target: kappa Light Chain

Background: HCAK1, Ig Kappa Chain C Region, IGKC, Immunoglobulin KM

Molecular Weight: ~22.5 kDa

Gene ID: 3514, 449609