Goat anti-Human IgM (Chain mu) Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN619228

Quick Overview for Goat anti-Human IgM (Chain mu) Antibody (Alkaline Phosphatase (AP)) (ABIN619228)

Target: IgM

Fragment: F(ab')2 fragment

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC)

Isotype: IgG

Product Details

Binding Specificity: Chain mu

Reactivity: Human

Host: Goat

Cross-Reactivity: Human

Characteristics: Goat anti-human IgM (µ chain), ALP (alkaline phosphatase) conjugated, is a F(ab) '2 fragment of a secondary antibody which binds to human IgM in immunological assays. Antibodies are adsorbed against human IgG or IgA. Antibody solution may contain small amounts of intact IgG.
F(ab)'2 fragment

Purification: Affinity purification

Purity: > 90 % based on SDS-PAGE.

Application Details

Application Notes: 1:500-1:2000 for ELISA/western blot, 1:20-1: 2000 for immunohistochemistry (IHC), 1:50-1: 5000 for immunocytochemistry (ICC) and flow cytometry.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.

Storage: 4 °C

Storage Comment: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Target Details

Target: IgM

Target Type: Antibody