Sheep anti-Rabbit IgG (Heavy & Light Chain) Antibody (TRITC)

Catalog No. ABIN619446

Quick Overview for Sheep anti-Rabbit IgG (Heavy & Light Chain) Antibody (TRITC) (ABIN619446)

Target: IgG

Clonality: Polyclonal

Conjugate: TRITC

Application: Immunomicroscopy (IM), Flow Cytometry (FACS)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Rabbit

Host: Sheep

Purpose: Sheep anti-Rabbit IgG (H&L), TRITC conjugated

Purification: Immunogen affinity purified

Immunogen: Purified rabbit IgG (H&L)

Application Details

Application Notes: The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 1,1 mL of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

Buffer: Immunogen affinity purified sheep IgG.

Handling Advice: Prepare working dilution prior to use and discard after use. Do not store working dilution,Be sure to mix well but without foaming

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Target Details

Target: IgG

Target Type: Antibody

Background:

Background: Sheep anti-rabbit IgG (H&L) is a secondary antibody conjugated to TRITC, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L). Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm, Emax = 570 nm.

Additional information: Purity of this preparation is > 95 % based on SDS-PAGE. Antibody concentration is 1.0 mg/mL (E 1 % at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05 % (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins.