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Goat anti-Human IgA (Chain alpha) Antibody (HRP)

ELISA, WB, IHC Polyclonal IgG HRP F(ab')2 fragment
Catalog No. ABIN619567

Quick Overview for Goat anti-Human IgA (Chain alpha) Antibody (HRP) (ABIN619567)

Target

IgA

Fragment

F(ab')2 fragment

Clonality

  • 307
  • 72
Polyclonal

Conjugate

  • 106
  • 54
  • 50
  • 40
  • 24
  • 18
  • 9
  • 6
  • 6
  • 6
  • 6
  • 5
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
HRP

Application

  • 193
  • 144
  • 129
  • 107
  • 107
  • 104
  • 61
  • 47
  • 47
  • 34
  • 28
  • 26
  • 23
  • 18
  • 16
  • 16
  • 14
  • 7
  • 7
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Isotype

IgG
  • Binding Specificity

    • 69
    • 61
    • 37
    • 18
    • 15
    • 13
    • 8
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Chain alpha

    Reactivity

    • 188
    • 61
    • 45
    • 16
    • 15
    • 13
    • 13
    • 12
    • 12
    • 11
    • 9
    • 6
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Human

    Host

    • 200
    • 109
    • 58
    • 17
    • 14
    • 6
    • 2
    Goat

    Predicted Reactivity

    human IgA heavy chain (alpha) F(ab)'2 fragment

    Characteristics

    Goat anti-human IgA heavy (alpha chain) is a secondary antibody conjugated to HRP which binds to human IgA heavy (alpha chain) F(ab) '2 fragments in immunological assays.

    Purification

    Affinity purification

    Immunogen

    purified human IgA, heavy (alpha chain)
  • Application Notes

    optimal working dilution should be determined by the investigator

    Comment

    No reactivity is observed to the light chains or non-immunoglobulin human serum proteins.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    For reconstitution please add 0.55 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.

    Handling Advice

    Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
    Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
    A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.

    Storage

    4 °C

    Storage Comment

    Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
  • Target

    IgA

    Target Type

    Antibody
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