Goat anti-Human IgE (Chain epsilon) Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN619576

Quick Overview for Goat anti-Human IgE (Chain epsilon) Antibody (Alkaline Phosphatase (AP)) (ABIN619576)

Target: IgE

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Isotype: IgG

Product Details

Binding Specificity: Chain epsilon

Reactivity: Human

Host: Goat

Cross-Reactivity: Sheep (Ovine), Mouse (Murine), Rabbit

Predicted Reactivity: human IgE heavy (epsilon chain)

Characteristics: Goat anti-human IgE heavy (epsilon chain) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgE heavy (epsilon chain) in immunological assays.

Purification: Affinity purification

Immunogen: purified human IgE

Application Details

Application Notes: 1: 500 - 2 000 (ELISA) and (WB), 1: 20 -1: 2000 (IHC), 1: 50 - 1: 5000 (ICC)

Comment:

No reactivity is observed to light chains or non-IgE human serum proteins based in immunoelectrophoresisno reactivity to bovine, mouse or rabbit serum proteins based on immunoelectrophoresis

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.

Storage: 4 °C

Storage Comment: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Target Details

Target: IgE