Goat anti-Human IgM (Chain mu) Antibody (HRP)

Catalog No. ABIN619752

Quick Overview for Goat anti-Human IgM (Chain mu) Antibody (HRP) (ABIN619752)

Target: IgM

Fragment: F(ab')2 fragment

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: Chain mu

Reactivity: Human

Host: Goat

Cross-Reactivity: Human

Characteristics: Goat anti-human IgM, µ chain, F(ab) '2 fragment is a HRP conjugated secondary antibody which is reacting with human IgM, µ chain, F(ab) '2. Antibody solution may contain small amounts of intact IgG.
F(ab)'2 fragment

Purification: Affinity purification

Purity: > 90 % based on SDS-PAGE.

Application Details

Application Notes: 1:500-1:5000 for immunohistochemistry (IHC), 1:10 000-1:50 000 for ELISA/western blot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution please add 0.55 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.

Handling Advice: Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.

Storage: 4 °C

Storage Comment: Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Target Details

Target: IgM

Target Type: Antibody