Rabbit anti-Human IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN637285

Quick Overview for Rabbit anti-Human IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) (ABIN637285)

Target: IgG

Conjugate: Alkaline Phosphatase (AP)

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Human

Host: Rabbit

Specificity: This antibody reacts with heavy (gamma) chains on human IgG and light chains on all human immunoglobulins.

Cross-Reactivity (Details): No reactivity is observed to non-immunoglobulin human serum proteins.

Characteristics: Rabbit anti Human IgG (H + L) secondary antibody (Alk Phos)
Alternative Names: Rabbit anti Human Immunoglobulin G (H + L) secondary antibody (Alk Phos)
Physical state: Clear, colorless liquid

Purification: Affinity purified using solid phase antibodies

Purity: > 95 % pure

Immunogen: Purified Human IgG (H&L)

Application Details

Application Notes: 1:5000-1:50,000 ELISA/WB, 1:500-1:2000 IHC

Comment:

Country of Origin: Rabbit serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 30 mM Triethanolamine, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA and 0.05 % (w/v) Sodium Azide. Protease/IgG free, pH 7.2.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted liquid at 2-8 °C. For storage at -20 °C, aliquot and dilute with an equal volume of glycerol to prevent loss of enzymatic activity.

Target Details

Target: IgG

Target Type: Antibody