Goat anti-Chicken IgG Antibody (Cy2) - Preadsorbed

Catalog No. ABIN6698801

Supplier Product No.: 603-111-126

Product Details

Target: IgG

Reactivity: Chicken

Host: Goat

Clonality: Polyclonal

Conjugate: Cy2

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Supplier Product No.: 603-111-126

Supplier: Rockland

Purpose: Chicken IgG (H&L) Antibody CY2 Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins

Characteristics: goat anti-Chicken IgG Antibody CY2 Conjugation, goat anti-Chicken IgG CY2 Conjugated Antibody, goat anti-Chicken IgY CY2,Anti-Chicken IgG Cy2 Antibody generated in goat detects chicken IgY.

Purification: Preadsorption: Pre-Adsorbed

Immunogen: Chicken IgG whole molecule

Isotype: IgG

Labeling Ratio: 5.81

Application Details

Application Notes:

FLISA_Dilution: 1:10,000 - 1:50,000

ELISA_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: User Optimized

IF_Microscopy_Dilution: 1:1,000 - 1:5,000

Western_Blot_Dilution: User Optimized

Other: FLOW CYTOMETRY 1:500 - 1:2,500

Comment:

Cy2 Conjugated secondary antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Cy2 Conjugated secondary antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.