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Rabbit anti-Sheep IgG Antibody (Cy5)

FACS, FLISA, FM Polyclonal IgG Cy5 F(ab')2 fragment
Rockland
Catalog No. ABIN6698834
Supplier Product No.: 713-410-002
  • Target See all IgG products
    IgG
    Fragment
    F(ab')2 fragment
    Reactivity
    • 730
    • 693
    • 596
    • 462
    • 399
    • 240
    • 207
    • 175
    • 145
    • 137
    • 112
    • 107
    • 106
    • 101
    • 82
    • 58
    • 47
    • 28
    • 27
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Sheep
    Host
    • 1762
    • 1546
    • 567
    • 258
    • 112
    • 111
    • 32
    • 31
    • 21
    • 18
    • 15
    • 11
    • 11
    Rabbit
    Clonality
    • 3390
    • 179
    • 16
    • 1
    Polyclonal
    Conjugate
    • 591
    • 421
    • 421
    • 378
    • 274
    • 262
    • 235
    • 118
    • 106
    • 72
    • 67
    • 64
    • 61
    • 58
    • 58
    • 50
    • 44
    • 43
    • 39
    • 39
    • 39
    • 37
    • 35
    • 35
    • 32
    • 31
    • 31
    • 30
    • 29
    • 29
    • 29
    • 29
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 27
    • 26
    • 26
    • 25
    • 21
    • 21
    • 19
    • 17
    • 17
    • 16
    Cy5
    Application
    • 2059
    • 1985
    • 1849
    • 1805
    • 998
    • 919
    • 895
    • 487
    • 424
    • 234
    • 234
    • 149
    • 98
    • 84
    • 65
    • 56
    • 51
    • 30
    • 28
    • 25
    • 20
    • 15
    • 11
    • 9
    • 8
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    Supplier Product No.
    713-410-002
    Supplier
    Rockland
    Purpose
    F(ab')2 Sheep IgG (H&L) Antibody Cy5 Conjugated
    Characteristics
    Rabbit F(ab')2 Anti-Sheep IgG CY5 Conjugated Antibody, Rabbit Fab2 Anti-Sheep IgG Antibody CY5 Conjugation,F(ab')2 Anti-Sheep IgG (H&L) Antibody generated in rabbit detects sheep IgG. Representing approximately 75 % of serum immunoglobulins, F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunofluorescence, IHC, and other immunoassays. This F(ab')2 Anti-Sheep IgG Antibody is conjugated to CY5.
    Immunogen
    Sheep IgG whole molecule
    Isotype
    IgG
    Labeling Ratio
    21
  • Application Notes

    FLISA_Dilution: 1:10,000 - 1:50,000

    Flow_Cytometry_Dilution: 1:500 - 1:2,500

    IF_Microscopy_Dilution: 1:1,000 - 1:5,000

    Other: FLOW CYTOMETRY 1:500 - 1:2,500

    Comment

    This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 250 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background
    IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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