Goat anti-Human IgG Antibody (DyLight 549) - Preadsorbed

Catalog No. ABIN6698927

Supplier Product No.: 609-142-123

Quick Overview for Goat anti-Human IgG Antibody (DyLight 549) - Preadsorbed (ABIN6698927)

Target: IgG

Clonality: Polyclonal

Conjugate: DyLight 549

Application: ELISA, Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Isotype: IgG

Product Details

Reactivity: Human

Host: Goat

Supplier Product No.: 609-142-123

Supplier: Rockland

Purpose: Human IgG (H&L) Antibody DyLight™ 549 Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Ms Rb Rt & Sh Serum Proteins

Characteristics: Goat Anti Human IgG DyLight 549™ Conjugated Antibody, Goat Anti-Human IgG Antibody DyLight 549™ conjugation,Anti-Human IgG (H&L) DyLight 549 generated in goat detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present.

Purification: Preadsorption: Pre-Adsorbed

Immunogen: Human IgG whole molecule

Labeling Ratio: 4.9

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Comment:

Anti-Human IgG (H&L) DyLight 549 has been tested by ELISA and dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: WB

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Stork, Ellenberger, Ruprecht, Reindl, Beißbarth, Friede, Kümpfel, Gerdes, Gloth, Liman, Paul, Brück, Metz: "Antibody signatures in patients with histopathologically defined multiple sclerosis patterns." in: Acta neuropathologica, Vol. 139, Issue 3, pp. 547-564, (2021) (PubMed).

Wallménius, Påhlson, Nilsson: "Retrospective serological study of Rickettsia spp. and Borrelia spp. antibodies in patients with peripheral facial nerve palsy." in: Infection ecology & epidemiology, Vol. 11, Issue 1, pp. 1987058, (2021) (PubMed).

Lindblom, Wallménius, Sjöwall, Fryland, Wilhelmsson, Lindgren, Forsberg, Nilsson: "Prevalence of Rickettsia spp. in Ticks and Serological and Clinical Outcomes in Tick-Bitten Individuals in Sweden and on the Åland Islands." in: PLoS ONE, Vol. 11, Issue 11, pp. e0166653, (2017) (PubMed).

Metz, Beißbarth, Ellenberger, Pache, Stork, Ringelstein, Aktas, Jarius, Wildemann, Dihazi, Friede, Brück, Ruprecht, Paul: "Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis." in: Neurology(R) neuroimmunology & neuroinflammation, Vol. 3, Issue 2, pp. e204, (2016) (PubMed).

Target Details

Target: IgG

Target Type: Antibody

Background: It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.