Goat anti-Human IgM Antibody (DyLight 649)

Catalog No. ABIN6698961

Supplier Product No.: 609-143-007

Quick Overview for Goat anti-Human IgM Antibody (DyLight 649) (ABIN6698961)

Target: IgM

Clonality: Polyclonal

Conjugate: DyLight 649

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Isotype: IgG

Product Details

Reactivity: Human

Host: Goat

Supplier Product No.: 609-143-007

Supplier: Rockland

Purpose: Human IgM (mu chain) Antibody DyLight™ 649 Conjugated

Characteristics: Goat Anti-Human IgM (mu chain) Antibody DyLight™ 649 Conjugated, Goat Anti Human IgM (mu chain) Antibody DyLight™ 649 Conjugated,Anti-Human IgM (mu heavy chain) DyLight 649 generated in goat detects specifically Human IgM mu heavy chain.

Immunogen: Human IgM whole molecule

Labeling Ratio: 1.8

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Comment:

The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. Anti-Human IgM (mu heavy chain) DyLight 649 is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Ishida, Corrigan, Chen, Liu, Kim, Song, Rutledge, Magee, LaBaer, Lowary, Lin, Achkar: "Mucosal and systemic antigen-specific antibody responses correlate with protection against active tuberculosis in nonhuman primates." in: EBioMedicine, Vol. 99, pp. 104897, (2024) (PubMed).

Target Details

Target: IgM

Target Type: Antibody

Background: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Anti-Human IgM mu chain antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.