Donkey anti-Mouse IgG Antibody (Cy2) - Preadsorbed

Catalog No. ABIN6698985

Supplier Product No.: 610-711-124

Product Details

Target: IgG

Reactivity: Mouse

Host: Donkey

Clonality: Polyclonal

Conjugate: Cy2

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Supplier Product No.: 610-711-124

Supplier: Rockland

Purpose: Mouse IgG (H&L) Antibody CY2 Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins

Characteristics: Donkey Anti-Mouse IgG (H&L) Antibody CY2 Conjugated Pre-Adsorbed, Donkey Anti Mouse IgG Antibody CY2 Conjugated,Anti-Mouse IgG Cy2 Antibody generated in donkey detects reactivity to Mouse IgG.

Purification: Preadsorption: Pre-Adsorbed

Immunogen: Mouse IgG whole molecule

Isotype: IgG

Labeling Ratio: 3.0

Application Details

Application Notes:

FLISA_Dilution: 1:10,000 - 1:50,000

ELISA_Dilution: 1:10,000 - 1:50,000

Flow_Cytometry_Dilution: 1:500 - 1:2,500

IF_Microscopy_Dilution: 1:1,000 - 1:5,000

Western_Blot_Dilution: User Optimized

Comment:

Cy2 Conjugated Secondary Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Suggested Applications: IF

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Tibshirani, Zhao, Gentil, Minotti, Marques, Keith, Rogaeva, Zinman, Rouaux, Robertson, Durham: "Dysregulation of chromatin remodelling complexes in amyotrophic lateral sclerosis." in: Human molecular genetics, Vol. 26, Issue 21, pp. 4142-4152, (2018) (PubMed).

Tibshirani, Tradewell, Mattina, Minotti, Yang, Zhou, Strong, Hayward, Durham: "Cytoplasmic sequestration of FUS/TLS associated with ALS alters histone marks through loss of nuclear protein arginine methyltransferase 1." in: Human molecular genetics, Vol. 24, Issue 3, pp. 773-86, (2015) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.