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Donkey anti-Mouse IgG Antibody (Cy2) - Preadsorbed

Rockland 610-711-124 ELISA, WB, FACS, FLISA, FM Polyclonal IgG Cy2
Rockland
Catalog No. ABIN6698985
Supplier Product No.: 610-711-124
  • Target See all IgG products
    IgG
    Reactivity
    • 726
    • 693
    • 595
    • 465
    • 393
    • 240
    • 208
    • 176
    • 145
    • 137
    • 112
    • 106
    • 105
    • 101
    • 82
    • 58
    • 47
    • 28
    • 27
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 1767
    • 1547
    • 568
    • 242
    • 112
    • 111
    • 32
    • 31
    • 21
    • 18
    • 15
    • 11
    • 11
    Donkey
    Clonality
    • 3395
    • 175
    • 4
    • 1
    Polyclonal
    Conjugate
    • 584
    • 421
    • 418
    • 378
    • 274
    • 262
    • 235
    • 119
    • 106
    • 73
    • 67
    • 64
    • 61
    • 58
    • 58
    • 50
    • 44
    • 43
    • 40
    • 39
    • 39
    • 37
    • 35
    • 32
    • 32
    • 31
    • 30
    • 29
    • 29
    • 29
    • 29
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 27
    • 26
    • 26
    • 25
    • 22
    • 21
    • 19
    • 17
    • 17
    • 16
    Cy2
    Application
    • 2043
    • 1987
    • 1839
    • 1792
    • 986
    • 919
    • 895
    • 487
    • 424
    • 234
    • 234
    • 151
    • 99
    • 84
    • 65
    • 56
    • 51
    • 30
    • 28
    • 25
    • 20
    • 15
    • 11
    • 9
    • 8
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    Supplier Product No.
    610-711-124
    Supplier
    Rockland
    Purpose
    Mouse IgG (H&L) Antibody CY2 Conjugated Pre-Adsorbed
    Cross-Reactivity (Details)
    Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins
    Characteristics
    Donkey Anti-Mouse IgG (H&L) Antibody CY2 Conjugated Pre-Adsorbed, Donkey Anti Mouse IgG Antibody CY2 Conjugated,Anti-Mouse IgG Cy2 Antibody generated in donkey detects reactivity to Mouse IgG.
    Purification
    Preadsorption: Pre-Adsorbed
    Immunogen
    Mouse IgG whole molecule
    Isotype
    IgG
    Labeling Ratio
    3.0
  • Application Notes

    FLISA_Dilution: 1:10,000 - 1:50,000

    ELISA_Dilution: 1:10,000 - 1:50,000

    Flow_Cytometry_Dilution: 1:500 - 1:2,500

    IF_Microscopy_Dilution: 1:1,000 - 1:5,000

    Western_Blot_Dilution: User Optimized

    Comment

    Cy2 Conjugated Secondary Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    Suggested Applications: IF

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Tibshirani, Zhao, Gentil, Minotti, Marques, Keith, Rogaeva, Zinman, Rouaux, Robertson, Durham: "Dysregulation of chromatin remodelling complexes in amyotrophic lateral sclerosis." in: Human molecular genetics, Vol. 26, Issue 21, pp. 4142-4152, (2018) (PubMed).

    Tibshirani, Tradewell, Mattina, Minotti, Yang, Zhou, Strong, Hayward, Durham: "Cytoplasmic sequestration of FUS/TLS associated with ALS alters histone marks through loss of nuclear protein arginine methyltransferase 1." in: Human molecular genetics, Vol. 24, Issue 3, pp. 773-86, (2015) (PubMed).

  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background
    Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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