Goat anti-Mouse IgG Antibody (DyLight 680) - Preadsorbed

Catalog No. ABIN6699032

Supplier Product No.: 610-144-121

Quick Overview for Goat anti-Mouse IgG Antibody (DyLight 680) - Preadsorbed (ABIN6699032)

Target: IgG

Clonality: Polyclonal

Conjugate: DyLight 680

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Goat

Supplier Product No.: 610-144-121

Supplier: Rockland

Purpose: Mouse IgG (H&L) Antibody DyLight™ 680 Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins

Characteristics: Goat Anti-Mouse IgG Secondary Antibody DyLight™680 Conjugated, Goat Anti-Mouse IgG Antibody DyLight™680 Conjugated, Anti-mouse IgG secondary antibody, anti-mouse IgG DyLight™680 conjugated secondary antibody,Anti-Mouse IgG DyLight 680 Antibody generated in goat detects reactivity to Mouse IgG.

Purification: Preadsorption: Pre-Adsorbed

Immunogen: Mouse IgG whole molecule

Labeling Ratio: 2.1

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Comment:

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: IF, IHC, Microarray, Multiplex, WB

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Szewczyk, Günther, Japtok, Frech, Naumann, Lee, Hermann: "FUS ALS neurons activate major stress pathways and reduce translation as an early protective mechanism against neurodegeneration." in: Cell reports, Vol. 42, Issue 2, pp. 112025, (2023) (PubMed).

Navarro, Tapia-Galisteo, Martín-García, Tarín, Corbacho, Gómez-López, Sánchez-Tirado, Campuzano, González-Cortés, Yáñez-Sedeño, Compte, Álvarez-Vallina, Sanz: "TGF-β-induced IGFBP-3 is a key paracrine factor from activated pericytes that promotes colorectal cancer cell migration and invasion." in: Molecular oncology, Vol. 14, Issue 10, pp. 2609-2628, (2021) (PubMed).

Fu, Cao, Schäfer, Stephan, Braspenning-Wesch, Schmitt, Bischoff, Müller, Schäfer, Vinzón, Rösl, Hasche: "Expression of different L1 isoforms of Mastomys natalensis papillomavirus as mechanism to circumvent adaptive immunity." in: eLife, Vol. 9, (2021) (PubMed).

Xue, Liu, Zhang, Ding, Shen, Shao, Wei: "Caffeine improves bladder function in diabetic rats via a neuroprotective effect." in: Experimental and therapeutic medicine, Vol. 21, Issue 5, pp. 501, (2021) (PubMed).

Schütter, Giavalisco, Brodesser, Graef: "Local Fatty Acid Channeling into Phospholipid Synthesis Drives Phagophore Expansion during Autophagy." in: Cell, Vol. 180, Issue 1, pp. 135-149.e14, (2020) (PubMed).

Mitra, Bodor, David, Abdul-Zani, Mata, Neumann, Reither, Tischer, Jansen: "Genetic screening identifies a SUMO protease dynamically maintaining centromeric chromatin." in: Nature communications, Vol. 11, Issue 1, pp. 501, (2020) (PubMed).

Espuny-Camacho, Arranz, Fiers, Snellinx, Ando, Munck, Bonnefont, Lambot, Corthout, Omodho, Vanden Eynden, Radaelli, Tesseur, Wray, Ebneth, Hardy, Leroy, Brion, Vanderhaeghen, De Strooper: "Hallmarks of Alzheimer's Disease in Stem-Cell-Derived Human Neurons Transplanted into Mouse Brain." in: Neuron, Vol. 93, Issue 5, pp. 1066-1081.e8, (2017) (PubMed).

Target Details

Target: IgG

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.