Donkey anti-Mouse IgG Antibody (DyLight 680) - Preadsorbed

Catalog No. ABIN6699034

Supplier Product No.: 610-744-124

Quick Overview for Donkey anti-Mouse IgG Antibody (DyLight 680) - Preadsorbed (ABIN6699034)

Target: IgG

Clonality: Polyclonal

Conjugate: DyLight 680

Application: ELISA, Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Donkey

Supplier Product No.: 610-744-124

Supplier: Rockland

Purpose: Mouse IgG (H&L) Antibody DyLight™ 680 Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins

Characteristics: Donkey anti-Mouse IgG DyLight 680™ Conjugated Antibody, Donkey anti Mouse IgG Antibody DyLight 680™ Conjugation,Anti-Mouse IgG DyLight680 Antibody generated in donkey detects reactivity to Mouse IgG.

Purification: Preadsorption: Pre-Adsorbed

Immunogen: Mouse IgG whole molecule

Labeling Ratio: 2.0

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Comment:

Anti-Mouse IgG DyLight680 Antibody has been tested by ELISA and dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: IF, WB

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Tehrani, Mikulski, Abdul-Zani, Mata, Siwek, Jansen: "STAT1 is required to establish but not maintain interferon-γ-induced transcriptional memory." in: The EMBO journal, Vol. 42, Issue 14, pp. e112259, (2023) (PubMed).

van den Berg, East, Mitra, Jansen: "p97/VCP drives turnover of SUMOylated centromeric CCAN proteins and CENP-A." in: Molecular biology of the cell, Vol. 34, Issue 5, pp. br6, (2023) (PubMed).

Fritz, Ranganathan, Wang, Hogg: "An alternative UPF1 isoform drives conditional remodeling of nonsense-mediated mRNA decay." in: The EMBO journal, Vol. 41, Issue 10, pp. e108898, (2022) (PubMed).

Navarro, Tapia-Galisteo, Martín-García, Tarín, Corbacho, Gómez-López, Sánchez-Tirado, Campuzano, González-Cortés, Yáñez-Sedeño, Compte, Álvarez-Vallina, Sanz: "TGF-β-induced IGFBP-3 is a key paracrine factor from activated pericytes that promotes colorectal cancer cell migration and invasion." in: Molecular oncology, Vol. 14, Issue 10, pp. 2609-2628, (2021) (PubMed).

Raimo, Zura-Miller, Fezelinia, Spruce, Zakopoulos, Mohsen, Vockley, Ischiropoulos: "Mitochondrial morphology, bioenergetics and proteomic responses in fatty acid oxidation disorders." in: Redox biology, Vol. 41, pp. 101923, (2021) (PubMed).

Siwek, Tehrani, Mata, Jansen: "Activation of Clustered IFNγ Target Genes Drives Cohesin-Controlled Transcriptional Memory." in: Molecular cell, Vol. 80, Issue 3, pp. 396-409.e6, (2021) (PubMed).

Bobkov, Huang, van den Berg, Mitra, Anselm, Lazou, Schunter, Feederle, Imhof, Lusser, Jansen, Heun: "Spt6 is a maintenance factor for centromeric CENP-A." in: Nature communications, Vol. 11, Issue 1, pp. 2919, (2020) (PubMed).

Target Details

Target: IgG

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.