Goat anti-Mouse IgG (Fc Region) Antibody (DyLight 800)

Catalog No. ABIN6699050

Supplier Product No.: 610-145-003

Quick Overview for Goat anti-Mouse IgG (Fc Region) Antibody (DyLight 800) (ABIN6699050)

Target: IgG

Clonality: Polyclonal

Conjugate: DyLight 800

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Isotype: IgG

Product Details

Binding Specificity: Fc Region

Reactivity: Mouse

Host: Goat

Supplier Product No.: 610-145-003

Supplier: Rockland

Purpose: Mouse IgG Fc Antibody DyLight™ 800 Conjugated

Characteristics: Goat Anti Mouse IgG F(c) Antibody DyLight™ 800 Conjugated, Goat Anti-Mouse IgG Fc Antibody DyLight™ 800 Conjugated, Goat Anti Mouse IgG Fc Fragment Antibody DyLight™ 800 Conjugated,Anti-Mouse IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH .

Immunogen: Mouse IgG F(c) fragment

Labeling Ratio: 2.3

Application Details

Application Notes:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Comment:

Anti-Mouse IgG F(c) DyLight 800 has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: IF, WB

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References

Szewczyk, Günther, Japtok, Frech, Naumann, Lee, Hermann: "FUS ALS neurons activate major stress pathways and reduce translation as an early protective mechanism against neurodegeneration." in: Cell reports, Vol. 42, Issue 2, pp. 112025, (2023) (PubMed).

Greenstein, Ng, Barrales, Tan, Braun, Al-Sady: "Local chromatin context regulates the genetic requirements of the heterochromatin spreading reaction." in: PLoS genetics, Vol. 18, Issue 5, pp. e1010201, (2022) (PubMed).

White, Milad, Sellers, Bernatchez: "Effect of Dysferlin Deficiency on Atherosclerosis and Plasma Lipoprotein Composition Under Normal and Hyperlipidemic Conditions." in: Frontiers in physiology, Vol. 12, pp. 675322, (2021) (PubMed).

Wang, Zhu, Shan, Wang, Yin, Yang, Wang, Zhang, Wang, Liang, Zhang, Jiang, Dong, Jiang, Qi, Feng: "Spy1, a unique cell cycle regulator, alters viability in ALS motor neurons and cell lines in response to mutant SOD1-induced DNA damage." in: DNA repair, Vol. 74, pp. 51-62, (2019) (PubMed).

Meese, Cepeda, Gahlen, Adams, Ficner, Ricci, Heller, Reisinger, Herget: "Activity-Dependent Phosphorylation by CaMKIIδ Alters the Ca2+ Affinity of the Multi-C2-Domain Protein Otoferlin." in: Frontiers in synaptic neuroscience, Vol. 9, pp. 13, (2017) (PubMed).

Target Details

Target: IgG

Target Type: Antibody

Background: Receptors bind the Fc portion of mouse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.