Rabbit anti-Mouse IgG (Fc Region) Antibody (DyLight 800) - Preadsorbed

Catalog No. ABIN6699051

Supplier Product No.: 610-445-003

Quick Overview for Rabbit anti-Mouse IgG (Fc Region) Antibody (DyLight 800) - Preadsorbed (ABIN6699051)

Target: IgG

Clonality: Polyclonal

Conjugate: DyLight 800

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Isotype: IgG

Product Details

Binding Specificity: Fc Region

Reactivity: Mouse

Host: Rabbit

Supplier Product No.: 610-445-003

Supplier: Rockland

Characteristics:

Synonyms: Rabbit Anti-Mouse IgG F(c) Antibody DyLight™ 800 Conjugation, Rabbit Anti-Mouse IgG Fc Antibody DyLight™ 800 Conjugated, Rabbit Anti-Mouse IgG Fc Fragment DyLight™800 Conjugated Antibody

Background: Anti-Mouse IgG F(c) DyLight antibody generated in rabbit is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of mouse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.

Purification: Preadsorption: immunoaffinity chromatography using Mouse IgG coupled to agarose beads

Immunogen:

Immunogen: Anti-Mouse IgG F(c) was produced by repeated immunization with mouse IgG F(c) fragment in rabbit.

Immunogen Type: Native Protein

Labeling Ratio: 1.9

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Mouse IgG F(c) DyLight™800 Antibody is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

FLISA Dilution: >1:20,000

ELISA Dilution: 1:40,000 - 1:180,000

Western Blot Dilution: 1:2,000 - 1:10,000

IF Microscopy Dilution: >1:5,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody