Rat anti-Human IgA Antibody (Biotin)

Catalog No. ABIN6963995

Quick Overview for Rat anti-Human IgA Antibody (Biotin) (ABIN6963995)

Target: IgA

Clonality: Monoclonal

Conjugate: Biotin

Application: ELISA, ELISpot

Clone: MT57

Isotype: IgG2a

Product Details

Reactivity: Human

Host: Rat

Specificity: Fc part of native IgA.

Cross-Reactivity (Details): The monoclonal antibody cross-reacts with IgA from non-human primates.

Purification: Purified from in vitro cultures by protein G affinity chromatography.

Immunogen: Human IgA

Application Details

Application Notes: For enumeration of IgA (antigen-specific) producing cells using ELISpot with coated antigen.

Comment:

Biotinylated through reaction with a N-hydroxysuccinimide ester of biotin.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: supplied at 0.5 mg/mL in PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store product at 4-8°C or frozen at -20°C or below. Avoid repeated freezing/ thawing. The expiry date indicates how long unopened products, stored according to instructions, are recommended for use.

Expiry Date: 18 months

References

Kim, Liebowitz, Lin, Kasparek, Pasetti, Garg, Gottlieb, Trager, Tucker: "Safety and immunogenicity of an oral tablet norovirus vaccine, a phase I randomized, placebo-controlled trial." in: JCI insight, Vol. 3, Issue 13, (2019) (PubMed).

Klippert, Neumann, Stahl-Hennig: "Comparative phenotypical analysis of B cells in fresh and cryopreserved mononuclear cells from blood and tissue of rhesus macaques." in: Journal of immunological methods, Vol. 433, pp. 59-68, (2017) (PubMed).

Bankó, Pozsgay, Szili, Tóth, Gáti, Nagy, Rojkovich, Sármay: "Induction and Differentiation of IL-10-Producing Regulatory B Cells from Healthy Blood Donors and Rheumatoid Arthritis Patients." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 198, Issue 4, pp. 1512-1520, (2017) (PubMed).

Järnum, Bockermann, Runström, Winstedt, Kjellman: "The Bacterial Enzyme IdeS Cleaves the IgG-Type of B Cell Receptor (BCR), Abolishes BCR-Mediated Cell Signaling, and Inhibits Memory B Cell Activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 195, Issue 12, pp. 5592-601, (2016) (PubMed).

Kesa, Larsson, Ahlborg, Axelsson: "Comparison of ELISpot and FluoroSpot in the Analysis of Swine Flu-Specific IgG and IgA Secretion by in Vivo Activated Human B Cells." in: Cells, Vol. 1, Issue 2, pp. 27-34, (2014) (PubMed).

Target Details

Target: IgA

Target Type: Antibody

Gene ID: 3494, 3493