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Recombinant Mouse anti-Human IgG Antibody (HRP)

ELISA, WB, DB Monoclonal Recombinant Antibody HRP scFv fragment

Catalog No. ABIN7233210

Datasheet as PDF

Target See all IgG products

IgG

Antibody Type

Recombinant Antibody

Fragment

scFv fragment
Reactivity
715

681

565

434

393

232

199

172

137

137

112

105

103

95

82

61

43

28

27

16

15

10

5

4

4

4

3

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1
Human
Host
1712

1491

540

249

122

101

38

32

21

15

13

11

11
Mouse
Clonality
3292

198

16

1
Monoclonal
Conjugate
596

402

398

375

272

249

221

117

84

69

64

61

58

54

50

46

43

40

40

39

38

36

35

35

32

31

31

30

29

29

29

29

29

28

28

28

28

28

28

28

27

27

26

26

25

21

20

19

17

17

15
HRP
Application
2011

1977

1815

1745

946

875

868

442

375

233

233

148

98

75

57

47

37

33

29

28

25

20

15

11

9

8

7

5

5

4

4

4

3

2

2

1

1

1

1

1

1

1

1
ELISA, Western Blotting (WB), Dot Blot (DB)

Purpose

Anti-Human IgG scFv-HRP Antibody

Specificity

Mouse variable regions (produced in Nicotiana benthamiana plants)

Characteristics

This recombinantly produced secondary anti-human IgG scFv fused to HRP is optimised for western blot and ELISA use. The reddish/brown colour is common with HRP conjugated antibodies.

Purification

This product was purified using affinity chromatography (Ni-IMAC)

Purity

90-95 %

Immunogen

Human IgG, whole molecule
Mouse anti-Human IgG (Fc Region) antibody
Animal-Free ELISA, WB, FACS, IF Multiclonal IgG2a Recombinant Antibody unconjugated

Mouse anti-Human IgG (Fc Region) antibody (HRP)
ELISA, IHC, WB Monoclonal JDC-10 IgG1 HRP

Goat anti-Human IgG antibody (PE)
ELISA, FACS Polyclonal IgG PE F(ab')2 fragment

Mouse anti-Human IgG (Fc Region) antibody (APC)
ELISA, FACS Monoclonal H2 IgG2b APC

Mouse anti-Human IgG (Fc Region) antibody (HRP)
Animal-Free ELISA, WB, FACS, IF Multiclonal IgG2a Recombinant Antibody HRP

Goat anti-Human IgG (Heavy & Light Chain) antibody
ELISA, IHC, WB Polyclonal IgG unconjugated

Goat anti-Human IgG (Fc Region) antibody (Biotin)
ELISA, FLISA Polyclonal IgG Biotin

Rabbit anti-Human IgG (Heavy & Light Chain) antibody (Biotin)
ELISA, IHC, WB, FACS Polyclonal IgG Biotin

Goat anti-Human IgG (Heavy Chain) antibody (TRITC)
ELISA, FACS Polyclonal IgG TRITC

Goat anti-Human IgG (Heavy Chain) antibody (Texas Red (TR))
ELISA, FACS Polyclonal IgG Texas Red (TR)

Application Notes

Western blot: Suggested working dilution 1/1000-1/5000 to detect 1 μg antigen in 5 % milk. Suggested incubation conditions:1 hr at 37C/ 4C overnight.

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

Phosphate Buffered saline (0.1M) pH 7.4

Storage

4 °C,-20 °C

Storage Comment

Short term: 2-4°C. Long term: -20°C
Target

IgG

Abstract

IgG Products

Target Type

Antibody