Recombinant Mouse anti-Human IgG Antibody (HRP)
Quick Overview for Recombinant Mouse anti-Human IgG Antibody (HRP) (ABIN7233210)
Target
Antibody Type
Fragment
Clonality
Conjugate
Application
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Reactivity
- Human
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Host
- Mouse
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Purpose
- Anti-Human IgG scFv-HRP Antibody
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Specificity
- Mouse variable regions (produced in Nicotiana benthamiana plants)
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Characteristics
- This recombinantly produced secondary anti-human IgG scFv fused to HRP is optimised for western blot and ELISA use. The reddish/brown colour is common with HRP conjugated antibodies.
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Purification
- This product was purified using affinity chromatography (Ni-IMAC)
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Purity
- 90-95 %
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Immunogen
- Human IgG, whole molecule
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Mouse anti-Human IgG (Fc Region) antibody (APC)
ELISA, FACS Monoclonal H2 IgG2b APC
Rabbit anti-Human IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Human IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated
Goat anti-Human IgG (Heavy Chain) antibody (TRITC)ELISA, FACS Polyclonal IgG TRITC
Goat anti-Human IgG antibody (Biotin)ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment
Goat anti-Human IgG (Heavy Chain) antibody (beta-Gal)ELISA, WB Polyclonal IgG beta-Gal
Goat anti-Human IgG (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, WB Polyclonal IgG Alkaline Phosphatase (AP)
Mouse anti-Human IgG (Fc Region) antibody (SPRD)ELISA, FACS Monoclonal H2 IgG2b SPRD
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Application Notes
- Western blot: Suggested working dilution 1/1000-1/5000 to detect 1 μg antigen in 5 % milk. Suggested incubation conditions:1 hr at 37C/ 4C overnight.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Phosphate Buffered saline (0.1M) pH 7.4
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Storage
- 4 °C,-20 °C
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Storage Comment
- Short term: 2-4°C. Long term: -20°C
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- IgG
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Target Type
- Antibody
Target
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