Recombinant Alpaca anti-Chicken IgY Antibody (Atto 488)
-
- Target See all IgY products
- IgY
- Antibody Type
- Recombinant Antibody
- Fragment
- single-domain Antibody (sdAb)
-
Reactivity
- Chicken
-
Host
- Alpaca
- Expression System
- E.coli
-
Clonality
- Monoclonal
-
Conjugate
- Atto 488
-
Application
- Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunocytochemistry (ICC)
- Specificity
- Chicken IgY, does not cross-react with mouse, rabbit, guinea pig or rat immunglobulins
- Purification
- Produced in: E.coli
- Immunogen
- anti-Chicken is a species-specific FluoTag directed against immunoglobulins from Chicken.
- Clone
- 3G2
- Labeling Ratio
- Two site-specifically conjugated fluorophores per sdAb.
-
Chicken anti-Chicken IgY antibody (DyLight 633)
IF, WB Polyclonal IgY DyLight 633
Chicken anti-Chicken IgY antibody (DyLight 405)IF, WB Polyclonal IgY DyLight 405
Chicken anti-Chicken IgY antibody (DyLight 488)IF, WB Polyclonal IgY DyLight 488
Goat anti-Chicken IgY (Heavy & Light Chain) antibody (FITC)ELISA, IHC, FACS Polyclonal IgG FITC
Chicken anti-Chicken IgY antibody (DyLight 550)IF, WB Polyclonal IgY DyLight 550
Rabbit anti-Chicken IgY antibody (DyLight 488)IF Polyclonal IgG DyLight 488
Rabbit anti-Chicken IgY antibody (DyLight 549)IF Polyclonal IgG DyLight 549
-
- Application Notes
- IF: 1:500 (corresponding to 5 nM final concentration if the sdAb was reconstituted as proposed bellow)
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of single-domain antibody is 2.5 µM
- Buffer
- The single sdAb clone was lyophilized from PBS pH 7.4.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
- Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
-
- Target
- IgY
- Abstract
- IgY Products
- Target Type
- Antibody
- Background
- The immune repertoire of chickens is much simpler compared to the one of mammals, producing only one isotype of immunoglobulins (IgY) with no subclasses. The use of polyclonal antibodies raised in chicken has steadily grown over the last years, mainly due to the need to add a new “species” when performing multitarget immunofluorescence.
-
