Recombinant Alpaca anti-Chicken IgY Antibody (DBCO)

Catalog No. ABIN7272938

Quick Overview for Recombinant Alpaca anti-Chicken IgY Antibody (DBCO) (ABIN7272938)

Target: IgY

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Clonality: Monoclonal

Conjugate: DBCO

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 3G2

Product Details

Reactivity: Chicken

Host: Alpaca

Expression System: E.coli

Specificity: Chicken IgY, does not cross-react with mouse, rabbit, guinea pig or rat immunglobulins

Purification: Produced in: E.coli

Immunogen: sdAbs anti-chicken IgY binds strongly and specifically to all IgYs. The anti-Chicken IgY sdAb is a single-domain anitbody against Chicken IgY, that can be labeled or immobilized by the customer.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconsitute with 250 µL pure H20 for immidate use or with 250 µL 50 % glycerol for storage at -80 °C

Buffer: sdAbs are lyophilized from PBS pH 7.4.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Target Details

Target: IgY

Target Type: Antibody

Background: The immunorepertoire of chickens is much simpler compared to any other mammal, producing only one isotype of immunoglobulins (IgY) with no subclasses. The use of polyclonal antibodies raised in chicken has steadily grown over the last years, mainly due to the need to add a new “species” when performing multitarget immunofluorescence.