Recombinant Alpaca anti-Rabbit IgG Antibody (Biotin)

Catalog No. ABIN7272952

Quick Overview for Recombinant Alpaca anti-Rabbit IgG Antibody (Biotin) (ABIN7272952)

Target: IgG

Antibody Type: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Clonality: Monoclonal

Conjugate: Biotin

Application: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 10E10

Product Details

Reactivity: Rabbit

Host: Alpaca

Expression System: E.coli

Specificity: Rabbit IgG, does not cross-react with mouse, guinea pig or chicken immunoglobulins

Purification: Produced in: E.coli

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconsitute with 250 µL pure H20 for immidate use or with 250 µL 50 % glycerol for storage at -80 °C

Buffer: sdAbs are lyophilized from PBS pH 7.4.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Target Details

Target: IgG

Target Type: Antibody

Background: The immune repertoire of rabbits, like of many other mammals, consists of several immunoglobulin (Ig) isotypes (IgA, IgG, IgE, and IgM). However, compared to other species, rabbits possess only one type of IgG carrying either a lambda or kappa light chain. Historically, rabbit antibodies have been a rich source for polyclonal antibodies, which were employed in various fields of life sciences. They are employed either directly from animal sera, or as affinity purified reagents. In addition, advances in antibody engineering over the last decade enabled the generation of monoclonal rabbit antibodies. Independent of its clonality and irrespectively of its VH domain, our single domain antibodies (sdAbs, or Nanobodies) bind the constant domain (Fc) of any rabbit IgG specifically and with high affinity, making this secondary tool ideal for demanding imaging applications.