Mouse anti-Human IgG (Fab Region) Antibody (Biotin)

Catalog No. ABIN7540457

Quick Overview for Mouse anti-Human IgG (Fab Region) Antibody (Biotin) (ABIN7540457)

Target: IgG

Clonality: Monoclonal

Conjugate: Biotin

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Clone: 4A11

Isotype: IgG1, kappa

Product Details

Binding Specificity: Fab Region

Reactivity: Human

Host: Mouse

Purpose: Anti-Hu IgG (Fab) Biotin

Characteristics: The mouse monoclonal antibody 4A11 reacts with Fab fragment of human IgG. This antibody detects Fab fragments regardless light chain type.

Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Immunogen: Purified human IgG.

Application Details

Application Notes: Flow cytometry: Recommended dilution: 0.5-4 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details

Target: IgG

Target Type: Antibody

Background:

Description: Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75 % of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.

Other names: Immunoglobulin G Fab fragment

Gene ID: 35