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Mouse anti-Human IgM Antibody (Biotin)

ELISA, FACS, WB, ICC, ICFC Monoclonal CH2 IgG1 kappa Biotin
Catalog No. ABIN7540459

Quick Overview for Mouse anti-Human IgM Antibody (Biotin) (ABIN7540459)

Target

IgM

Clonality

  • 511
  • 91
Monoclonal

Conjugate

  • 119
  • 80
  • 75
  • 56
  • 33
  • 27
  • 26
  • 23
  • 13
  • 12
  • 11
  • 11
  • 11
  • 11
  • 11
  • 11
  • 11
  • 9
  • 9
  • 9
  • 9
  • 7
  • 7
  • 6
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
Biotin

Application

  • 350
  • 302
  • 295
  • 232
  • 163
  • 114
  • 91
  • 91
  • 64
  • 64
  • 36
  • 35
  • 26
  • 22
  • 20
  • 15
  • 9
  • 6
  • 5
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Flow Cytometry (FACS), Western Blotting (WB), Immunocytochemistry (ICC), Intracellular Flow Cytometry (ICFC)

Clone

CH2

Isotype

IgG1 kappa
  • Reactivity

    • 208
    • 159
    • 89
    • 40
    • 30
    • 28
    • 27
    • 22
    • 17
    • 13
    • 13
    • 13
    • 12
    • 12
    • 12
    • 4
    • 2
    • 1
    Human

    Host

    • 350
    • 217
    • 84
    • 20
    • 14
    • 5
    • 4
    • 1
    • 1
    Mouse

    Purpose

    Anti-Hu IgM Biotin

    Specificity

    The antibody CH2 reacts with Fc fragment of human IgM.

    Purification

    Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

    Immunogen

    Purified human IgM.
  • Application Notes

    Flow cytometry: Recommended dilution: 1-5 μg/mL.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C

    Storage Comment

    Store at 2-8°C. Do not freeze.
  • Target

    IgM

    Target Type

    Antibody

    Background

    Description: Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.

    Other names: immunoglobulin M

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