Recombinant Mouse anti-Human IgG (Fc Region) Antibody

Catalog No. ABIN7581782

Product Details

Target: IgG

Antibody Type: Recombinant Antibody

Binding Specificity: Fc Region

Reactivity: Human

Host: Mouse

Expression System: Phage display

Clonality: Multiclonal

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Purpose: Mouse anti-human IgG (Fc-specific) Antibody, animal-free multiclonal Ab

Specificity: This is an antibody developed by antibody phage display technology using a human naive antibody gene library and human IgG as antigen. For this antibody both the heavy and light chains are cloned and expressed, generating full-length antibodies.

No Cross-Reactivity: Cat, Cow, Dog, Goat, Horse, Human, Mouse, Rabbit, Rat

Cross-Reactivity (Details): No known cross reactivity. No cross-reactivity to human IgM, human IgE, human IgA, mouse IgG, rabbit IgG, horse IgG, bovine IgG, goat IgG, rat IgG, dog IgG, cat IgG. Other species were not tested.

Characteristics: Multiclonals are recombinant secondary antibodies that combine the best of polyclonal antisera and hybridoma monoclonal antibodies, while eliminating their disadvantages, plus they add the quality of recombinant reagents. Multiclonal antibodies consist of carefully adjusted mixtures recognizing different epitopes on all four different subclasses of human IgG. Their respective epitope binding sites do not compete with each other, therefore amplifying signal strengths. Multiclonals can contain up to 17 different individually tested monoclonal recombinant antibodies. This provides the typical advantage of polyepitope recognition which is key to the broad application profile of polyclonal antisera, but eliminates their disadvantages (limited batch sizes and batch-to-batch variations, no long-term reproducibility, undefined composition, unknown constituents). Multiclonals are completely sequence defined, implying their unlimited long-term availability and always identical test results. Their composition of individually characterised antibodies minimizes cross-reactivity with other targets, since they do not contain non-target directed IgG like all animal derived polyclonals do. This allows for a much lower unspecific binding reactivity in many assays compared to animal based products.

Purification: Protein A purification

Grade: Animal-Free

Immunogen: No immunization, animal-free antibody development. Antigen: human intravenous immunoglobulins (IVIg)

Isotype: IgG2a

Application Details

Application Notes: Western Blot: 0.2-5 μg/mL ELISA: 1-12 μg/mL as coating antibody 0.5-5 μg/mL as detection antibody IF: 0.1 - 10 μg/mL Optimal working dilution should be determined by the investigator

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4,

Storage: -20 °C

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Molecular Weight: 150 kDa

UniProt: P01857