Recombinant Mouse anti-Rabbit IgG Antibody (HRP)

Catalog No. ABIN7581784

Product Details

Target: IgG

Antibody Type: Recombinant Antibody

Reactivity: Rabbit

Host: Mouse

Expression System: Phage display

Clonality: Multiclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Purpose: recombinant Multiclonal anti-rabbit IgG

Specificity: This is an antibody developed by antibody phage display technology using a human naive antibody gene library and rabbit IgG antigen. For this antibody both the heavy and light chains are cloned and expressed, generating full-length antibodies.

No Cross-Reactivity: Goat, Human, Mouse

Cross-Reactivity (Details): No known cross reactivity. No cross reactivtiy to mouse IgG, human IgG, goat IgG. Other species were not tested.

Characteristics: Multiclonals are recombinant secondary antibodies that combine the best of polyclonal antisera and hybridoma monoclonal antibodies, while eliminating their disadvantages, plus they add the quality of recombinant reagents. Multiclonal antibodies consist of carefully adjusted mixtures recognizing different epitopes on all subclasses of rabbit IgG. Their respective epitope binding sites do not compete with each other, therefore amplifying signal strengths. Multiclonals can contain up to 17 different individually tested monoclonal recombinant antibodies. This provides the typical advantage of polyepitope recognition which is key to the broad application profile of polyclonal antisera, but eliminates their disadvantages (limited batch sizes and batch-to-batch variations, no long-term reproducibility, undefined composition, unknown constituents). Multiclonals are completely sequence defined, implying their unlimited long-term availability and always identical test results. Their composition of individually characterised antibodies minimizes cross-reactivity with other targets, since they do not contain non-target directed IgG like all animal derived polyclonals do. This allows for a much lower unspecific binding reactivity in many assays compared to animal based products.

Purification: Protein A purification

Grade: Animal-Free

Immunogen: No immunization, animal-free antibody development

Isotype: IgG2a

Application Details

Application Notes: Western Blot: 1:1,000 - 1:20,000 ELISA: 1:1,000 - 1:50,000 Optimal working dilution should be determined by the investigator

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, 0,02 % ProClin300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Molecular Weight: 150 kDa