Mouse anti-Human IgG (Heavy Chain) Antibody (CF®488A)

Catalog No. ABIN7879449

Quick Overview for Mouse anti-Human IgG (Heavy Chain) Antibody (CF®488A) (ABIN7879449)

Target: IgG

Clonality: Monoclonal

Conjugate: CF®488A

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: IG266

Isotype: IgG2a, kappa

Product Details

Binding Specificity: Heavy Chain

Reactivity: Human

Host: Mouse

Purpose: IgG Antibody

Purification: Protein G affinity chromatography

Immunogen: Purified human IgG heavy chain was used as the immunogen for this anti-IgG antibody.

Application Details

Application Notes: Optimal dilution of the IgG antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 500 µL at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the IgG antibody at 2-8oC, protected from light.

Target Details

Target: IgG

Target Type: Antibody

Background: Immunoglobulin gamma (IgG) is the most common class of antibody in blood and extracellular fluid. Approximately 75 % of serum antibodies in humans are IgG. There are four immunoglobulin gamma subclasses: one, two, three and four. IgG1 is the most common, with 68 % of all gamma class antibodies being G1, and G4 is the least common at 4 %. Gamma class antibodies are found primarily in the secondary immune response, class switching from IgM and IgD. They are the only class of antibody that can cross the placenta, and along with IgA secreted in breast milk, provide the neonate with humoral immunity before immune system development occurs.

This antibody recognizes a protein of 75 kDa identified as the gamma heavy chain of human immunoglobulins. It does not cross-react with alpha, mu, epsilon, or delta heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. The IgG antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.