Goat anti-Monkey IgA (Free & Bound), (Secretory Component) Antibody (Biotin)

Catalog No. ABIN784368

Quick Overview for Goat anti-Monkey IgA (Free & Bound), (Secretory Component) Antibody (Biotin) (ABIN784368)

Target: IgA

Clonality: Polyclonal

Conjugate: Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Dot Blot (DB)

Isotype: IgG

Product Details

Binding Specificity: Free & Bound, Secretory Component

Reactivity: Monkey

Host: Goat

Characteristics: Molar Ratio: Biotin / IgG aprox. 8.6

Purification: DEAE-column Chromatography

Immunogen: Purified free monkey secretory component isolated from pooled milk is used for immunization. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 1 mL sterile distilled water

Concentration: 10.0 mg/mL

Buffer: PBS, pH 7.2 No preservative added, as it may interfere with the antibody activity. No foreign proteins added.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.

Target Details

Target: IgA

Target Type: Antibody

Background: Secretory component is present in Monkey secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimmer IgA is transported into secretions by its binding to the SC on the epithelial cells. SC also has an affinity for polymeric IgM.Synonyms: Monkey Immunoglobulin A