Goat anti-Chimpanzee IgA (Free & Bound), (Secretory Component) Antibody (FITC)

Catalog No. ABIN784369

Quick Overview for Goat anti-Chimpanzee IgA (Free & Bound), (Secretory Component) Antibody (FITC) (ABIN784369)

Target: IgA

Clonality: Polyclonal

Conjugate: FITC

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Dot Blot (DB)

Isotype: IgG

Product Details

Binding Specificity: Free & Bound, Secretory Component

Reactivity: Monkey, Chimpanzee

Host: Goat

Cross-Reactivity: Monkey

Characteristics: Molar Ratio: 1,6

Purification: DEAE-column Chromatography

Immunogen: Secretory component is present in monkey secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. SC also has an affinity for polymeric IgM. Purified free monkey secretory component isolated from pooled milk is used for immunization. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Absorption / Emission: 492 nm/512 nm

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 1 mL sterile distilled water

Concentration: 10 mg/mL

Buffer: PBS, pH 7.2 No preservative added, as it may interfere with the antibody activity. No foreign proteins added.

Preservative: Without preservative

Handling Advice: Product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.

Target Details

Target: IgA

Target Type: Antibody

Background: Synonyms: Monkey Immunoglobulin A