Mouse anti-Human IgE Antibody

Catalog No. ABIN94399

Quick Overview for Mouse anti-Human IgE Antibody (ABIN94399)

Target: IgE

Clonality: Monoclonal

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Clone: 4H10

Isotype: IgG1

Product Details

Reactivity: Human

Host: Mouse

Purpose: Anti-Hu IgE Purified

Cross-Reactivity (Details): Human

Characteristics: The mouse monoclonal antibody 4H10 reacts with human IgE, it recognizes an epitope different from the ones recognized by BE5 and 4G7.325 antibodies to IgE. The epitope is located within the amino acids 267-279 (TWLEDGQVMDVDL).

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Purified human IgE.

Application Details

Application Notes: Flow cytometry: Recommended dilution: 4 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details

Target: IgE

Background: Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.,Immunoglobulin E

Molecular Weight: 180 kDa

Gene ID: 35