Mouse anti-Human IgG (Fd Region) Antibody

Catalog No. ABIN952843

Quick Overview for Mouse anti-Human IgG (Fd Region) Antibody (ABIN952843)

Target: IgG

Clonality: Monoclonal

Application: Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Clone: HP6045

Isotype: IgG1

Product Details

Binding Specificity: Fd Region

Reactivity: Human

Host: Mouse

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Protein G Chromatography.

Immunogen: Human IgG Fd

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.

References

Lebozec, Jandrot-Perrus, Avenard, Favre-Bulle, Billiald: "Design, development and characterization of ACT017, a humanized Fab that blocks platelet's glycoprotein VI function without causing bleeding risks." in: mAbs, Vol. 9, Issue 6, pp. 945-958, (2017) (PubMed).

Target Details

Target: IgG

Target Type: Antibody

Background: The Fd region of Human Immunoglobulins is located at the N-terminal part of the heavy chain and is an essential component of the antigen-binding fragment Fab. Fd, like the light chain, contains a C-terminal constant (CH1) and N- terminal variable (VH) domain. The hypervariable regions in both the light chain and Fd determine the specificity of the Fab.Synonyms: Human Immunoglobulin G