Rabbit anti-Dog IgG (Heavy & Light Chain) Antibody (HRP)

Catalog No. ABIN964822

Supplier Product No.: 304-4302

Quick Overview for Rabbit anti-Dog IgG (Heavy & Light Chain) Antibody (HRP) (ABIN964822)

Target: IgG

Fragment: F(ab')2 fragment

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Dog

Host: Rabbit

Supplier Product No.: 304-4302

Supplier: Rockland

Purpose: F(ab')2 Dog IgG (H&L) Antibody Peroxidase Conjugated

Cross-Reactivity (Details): The F(ab')2  fragment of a rabbit IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above.Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Dog IgG and Dog Serum.  No reaction was observed against anti-Rabbit IgG F(c) or anti-Pepsin.

Characteristics: F(ab')2 Anti-Dog IgG F(ab')2 Fluorescein Antibody generated in rabbit detects Dog F(ab')2. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

Purification: F(ab')2 Anti-DOG IgG Antibody is Peroxidase Conjugated.

Immunogen: Optional[Immunogen]: Dog IgG whole molecule

Application Details

Application Notes: Application Note: Dog antibody has been assayed against 1.0 μg of Dog IgG in a standard capture ELISA. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for anti-dog antibody.  F(ab')2 Anti-Dog IgG is suitable for ELISA, Western Blot and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:10,000 - 1:50,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody

Background: Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays. F(ab')2 Anti-Dog antibody is ideal for investigators involved in serum protein component research.