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Rabbit anti-Guinea Pig IgG (F(ab')2 Region) Antibody (FITC)

FACS, FLISA, FM Polyclonal IgG FITC F(ab')2 fragment
Rockland
Catalog No. ABIN964832
Supplier Product No.: 306-4204
  • Target See all IgG products
    IgG
    Fragment
    F(ab')2 fragment
    Binding Specificity
    • 2398
    • 511
    • 416
    • 146
    • 129
    • 128
    • 67
    • 29
    • 28
    • 28
    • 21
    • 9
    • 5
    • 5
    • 2
    • 1
    • 1
    F(ab')2 Region
    Reactivity
    • 683
    • 677
    • 569
    • 433
    • 375
    • 208
    • 194
    • 144
    • 127
    • 126
    • 106
    • 102
    • 95
    • 88
    • 82
    • 58
    • 47
    • 28
    • 27
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Guinea Pig
    Host
    • 1706
    • 1430
    • 525
    • 189
    • 112
    • 111
    • 32
    • 31
    • 21
    • 18
    • 15
    • 11
    • 11
    Rabbit
    Clonality
    • 3130
    • 168
    • 4
    • 1
    Polyclonal
    Conjugate
    • 580
    • 414
    • 413
    • 367
    • 274
    • 262
    • 235
    • 118
    • 85
    • 72
    • 67
    • 64
    • 61
    • 58
    • 58
    • 44
    • 39
    • 39
    • 37
    • 35
    • 32
    • 32
    • 31
    • 30
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 27
    • 26
    • 26
    • 25
    • 23
    • 21
    • 21
    • 19
    • 17
    • 17
    • 16
    • 15
    • 15
    • 15
    • 15
    • 13
    • 10
    • 8
    FITC
    Application
    • 2023
    • 1985
    • 1810
    • 1767
    • 986
    • 919
    • 895
    • 487
    • 424
    • 149
    • 98
    • 64
    • 56
    • 51
    • 45
    • 30
    • 28
    • 25
    • 20
    • 15
    • 11
    • 9
    • 8
    • 8
    • 8
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    Supplier Product No.
    306-4204
    Supplier
    Rockland
    Purpose
    F(ab')2 Guinea Pig IgG F(ab')2 Antibody Fluorescein Conjugated
    Cross-Reactivity (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Guinea Pig IgG, Guinea Pig IgG F(ab')2 and Guinea Pig Serum.  No reaction was observed against Guinea Pig IgG F(c), anti-Rabbit IgG F(c) or anti-Pepsin.
    Characteristics
    F(ab')2 Anti-Guinea Pig IgG Fluorescein Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.
    Purification
    This product is a F(ab')2  fragment of an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by extensive dialysis against the buffer stated above.
    Immunogen
    Optional[Immunogen]: Guinea Pig IgG F(ab')2 fragment
    Isotype
    IgG
  • Application Notes
    Application Note: F(ab')2 Anti-Guinea Pig IgG F(ab')2 Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 2.0 mL
    Concentration
    10.0 mg/mL
    Buffer

    Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    , Preservative:0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Expiry Date
    12 months
  • Target
    IgG
    Abstract
    IgG Products
    Target Type
    Antibody
    Background
    F(ab')2 Anti-Guinea Pig IgG F(ab')2 Fluorescein Antibody generated in rabbit detects Guinea Pig F(ab')2. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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