Donkey anti-Rat IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed

Catalog No. ABIN965276

Supplier Product No.: 712-702-149

Quick Overview for Donkey anti-Rat IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed (ABIN965276)

Target: IgG

Fragment: F(ab')2 fragment

Clonality: Polyclonal

Conjugate: FITC

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM), Dot Blot (DB)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Rat

Host: Donkey

Supplier Product No.: 712-702-149

Supplier: Rockland

Purpose: F(ab')2 Rat IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Donkey Serum, Rat IgG and Rat Serum.  No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.

Characteristics: F(ab')2 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Immunogen: Optional[Immunogen]: Rat IgG whole molecule

Application Details

Application Notes: Application Note: F(ab')2 Anti-Rat IgG Fluorescein Conjugated Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Flow Cytometry Dilution: User Optimized IF Microscopy Dilution: 1:500-1:2,500 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL

Concentration: 1.0 mg/mL

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody

Background: F(ab')2 Anti-Rat IgG (H&L) Antibody generated in donkey detects rat IgG. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunofluorescence, IHC, and other immunoassays. This F(ab')2 Anti-Rat IgG Antibody is conjugated to Fluorescein.