Rabbit anti-Mouse IgG (Heavy & Light Chain) Antibody (TRITC)

Catalog No. ABIN965362

Supplier Product No.: 810-4002

Quick Overview for Rabbit anti-Mouse IgG (Heavy & Light Chain) Antibody (TRITC) (ABIN965362)

Target: IgG

Fragment: Fab fragment

Clonality: Polyclonal

Conjugate: TRITC

Application: Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Rabbit

Supplier Product No.: 810-4002

Supplier: Rockland

Purpose: Fab Mouse IgG (H&L) Antibody Rhodamine Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum.  No reaction was observed against anti-Papain or anti-Rabbit IgG F(c).

Characteristics: Fab Anti-Mouse IgG (H&L) Texas Red Antibody generated in goat detects Mouse IgG. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.

Immunogen: Optional[Immunogen]: Mouse IgG whole molecule

Application Details

Application Notes: Application Note: This product is suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL

Concentration: 1.0 mg/mL

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody

Background: Fab Anti-Mouse IgG (H&L) Rhodamine Antibody generated in rabbit detects Mouse IgG. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Fab Antibody is ideal for investigators who routinely perform flow cytometry, immunofluorescence, IHC, and other immunoassays. This Fab Anti-Mouse IgG Antibody is conjugated to rhodamine.