Goat anti-Rabbit IgG (Chain gamma), (Heavy Chain) Antibody (DyLight 650)

Catalog No. ABIN969683

Quick Overview for Goat anti-Rabbit IgG (Chain gamma), (Heavy Chain) Antibody (DyLight 650) (ABIN969683)

Target: IgG

Conjugate: DyLight 650

Application: Flow Cytometry (FACS), Immunomicroscopy (IM)

Product Details

Binding Specificity: Chain gamma, Heavy Chain

Reactivity: Rabbit

Host: Goat

Specificity: Based on IEP, this antibody reacts with: heavy (gamma) chains on rabbit IgG

No Cross-Reactivity: Cow (Bovine), Horse (Equine), Human

Characteristics: Fluorophore: DyLight® 650 (Ex = 652 nm, Em = 672 nm). Fluor Protein Ratio: Moles DyLight® 650 per Mole Antibody.

Purification: Affinity purified using solid phase Rabbit IgG (H and L)

Purity: > 95 % based on SDS-PAGE

Immunogen: Purified Rabbit IgG Fc

Application Details

Application Notes: This conjugate is suitable for immunomicroscopy and flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications

Comment:

Country of Origin: Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Trademark: DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Rehydrate with 1.1 mL of deionized water. Allow reconstituted product to stand for at least 30 minutes at room temperature prior to dilution. If necessary, Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Concentration: 1.0 mg/mL

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Product is photosensitive and should be protected from light.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.

Storage: 4 °C

Target Details

Target: IgG

Target Type: Antibody